Gene:  NEIL3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al. 2002 [PubMed 12509226]).[supplied by OMIM Mar 2008]

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PrimePCR™ SYBR® Green Assay: NEIL3, Human
nei endonuclease VIII-like 3 (E. coli)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043285
Info:   Same primer pair as used in probe assay qHsaCEP0050547
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PrimePCR™ SYBR® Green Assay: NEIL3, Human
nei endonuclease VIII-like 3 (E. coli)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009588
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: NEIL3, Human
nei endonuclease VIII-like 3 (E. coli)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050547
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PrimePCR™ ddPCR™ Mutation Assay:NEIL3 p.L549L, Human
nei endonuclease VIII-like 3 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503914
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NEIL3 WT for p.L549L, Human
nei endonuclease VIII-like 3 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503915
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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