Gene:  NEB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes nebulin a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kD due to alternative splicing that is tissue- species-and developmental stage-specific. Of the 183 exons in the nebulin gene at least 43 are alternatively spliced although exons 143 and 144 are not found in the same transcript. Of the several thousand transcript variants predicted for nebulin the RefSeq Project has decided to create three representative RefSeq records. Mutations in this gene are associated with recessive nemaline myopathy. [provided by RefSeq Sep 2009]

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PrimePCR™ SYBR® Green Assay: NEB, Human
nebulin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011772
Info:   Same primer pair as used in probe assay qHsaCIP0028175
List Price:    $125.00
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PrimePCR™ Probe Assay: NEB, Human
nebulin

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028175
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NEB, Human
nebulin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018130
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011772; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NEB, Human
nebulin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050244
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028175; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NEB, Human
nebulin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050245
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028175; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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