Gene:  NDUFA5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing water soluble "iron-sulfur protein" (IP) fraction of complex I although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002394
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044224
Info:   Same primer pair as used in probe assay qHsaCEP0051486
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051486
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001111
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002394; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008900
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044224; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036112
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051486; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NDUFA5, Human
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036113
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051486; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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