Gene:  NCOA3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0018490
Info:   Same primer pair as used in probe assay qHsaCIP0031885
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PrimePCR™ Probe Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031885
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023297
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0018490; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500436
Info:   FAM; 67nt amplicon; exonic; FAM version of NCOA3 (dHsaCP2506555)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506555
Info:   HEX; 67nt amplicon; exonic; HEX version of NCOA3 (dHsaCP2500436)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056820
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031885; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: NCOA3, Human
nuclear receptor coactivator 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056821
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031885; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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