Gene:  NCOA2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The NCOA2 gene encodes nuclear receptor coactivator 2 which aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth development and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily which includes the 5 steroid receptors and class II nuclear receptors (see below) are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain a central DNA-binding domain and a C-terminal hormone-binding domain. Before the binding of hormone steroid receptors which are sometimes called class I of the nuclear hormone receptor family remain inactive in a complex with heat-shock protein-90 (MIM 140571) and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex bind as homodimers to specific DNA enhancer elements associated with target genes and modulate that gene's transcription. After binding to enhancer elements transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation (Hong et al. 1997 [PubMed 9111344]).[supplied by OMIM Nov 2010]

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PrimePCR™ SYBR® Green Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010767
Info:   Same primer pair as used in probe assay qHsaCIP0027721
List Price:    $125.00
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PrimePCR™ Probe Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027721
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5017369
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010767; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000113
Info:   FAM: 70nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2507118
Info:   HEX; 70nt amplicon; exonic; HEX version of NCOA2 (dHsaCP1000113)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5049470
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027721; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NCOA2, Human
nuclear receptor coactivator 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5049471
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027721; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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