Gene:  NCF4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox) which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NCF4, Human
neutrophil cytosolic factor 4, 40kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020999
Info:   Same primer pair as used in probe assay qHsaCIP0032863
List Price:    $130.00
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PrimePCR™ Probe Assay: NCF4, Human
neutrophil cytosolic factor 4, 40kDa

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0032863
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NCF4, Human
neutrophil cytosolic factor 4, 40kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023700
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020999; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NCF4, Human
neutrophil cytosolic factor 4, 40kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057224
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0032863; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NCF4, Human
neutrophil cytosolic factor 4, 40kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057225
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0032863; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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