Gene:  NARF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene including one with a novel exon that is generated by RNA editing. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NARF, Human
nuclear prelamin A recognition factor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009849
Info:   Same primer pair as used in probe assay qHsaCIP0027351
List Price:    $125.00
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PrimePCR™ Probe Assay: NARF, Human
nuclear prelamin A recognition factor

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027351
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NARF, Human
nuclear prelamin A recognition factor

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016669
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009849; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NARF, Human
nuclear prelamin A recognition factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048830
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027351; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NARF, Human
nuclear prelamin A recognition factor

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048831
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027351; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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