Gene:  NAGPA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide the protein's C-terminus contains multiple signals for trafficking it between lysosomes the plasma membrane and trans-Golgi network. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: NAGPA, Human
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016537
Info:   Same primer pair as used in probe assay qHsaCIP0030652
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PrimePCR™ Probe Assay: NAGPA, Human
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030652
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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