Gene:  MTIF2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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During the initiation of protein biosynthesis initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt) an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005319
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057031
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058136
List Price:    $130.00
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PrimePCR™ Probe Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058136
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5003286
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005319; Exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013614
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0057031; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045538
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0058136; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MTIF2, Human
mitochondrial translational initiation factor 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045539
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0058136; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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