Gene:  MSH4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor thus provoking ADP-ATP exchange and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq Aug 2011]

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PrimePCR™ SYBR® Green Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042166
Info:   Same primer pair as used in probe assay qHsaCEP0049429
List Price:    $138.00
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PrimePCR™ SYBR® Green Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016711
List Price:    $138.00
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PrimePCR™ Probe Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049429
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007123
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042166; exonic
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021902
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016711; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032558
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049429; exonic
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MSH4, Human
mutS homolog 4 (E. coli)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032559
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049429; exonic
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:MSH4 p.E742G, Human
mutS homolog 4 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504574
Info:   98nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:MSH4 WT for p.E742G, Human
mutS homolog 4 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504575
Info:   98nt amplicon; crosses exon-intron junction; in silico
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