Gene:  MRPS12, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MRPS12, Human
mitochondrial ribosomal protein S12

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0038880
Info:   Same primer pair as used in probe assay qHsaCIP0041602
List Price:    $125.00
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PrimePCR™ Probe Assay: MRPS12, Human
mitochondrial ribosomal protein S12

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0041602
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MRPS12, Human
mitochondrial ribosomal protein S12

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5025276
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0038880; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPS12, Human
mitochondrial ribosomal protein S12

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059238
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0041602; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPS12, Human
mitochondrial ribosomal protein S12

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059239
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0041602; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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