Gene:  MRPL23, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed despite its location within a region of imprinted genes on chromosome 11. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048608
Info:   Same primer pair as used in probe assay qHsaCEP0055868
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PrimePCR™ SYBR® Green Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014500
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PrimePCR™ Probe Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055868
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012809
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048608; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020105
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014500; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043928
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055868; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPL23, Human
mitochondrial ribosomal protein L23

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043929
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055868; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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