Gene:  MRPL20, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MRPL20, Human
mitochondrial ribosomal protein L20

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0020493
Info:   Same primer pair as used in probe assay qHsaCEP0032767
List Price:    $125.00
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PrimePCR™ Probe Assay: MRPL20, Human
mitochondrial ribosomal protein L20

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0032767
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MRPL20, Human
mitochondrial ribosomal protein L20

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5004771
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0020493; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPL20, Human
mitochondrial ribosomal protein L20

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029038
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0032767; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MRPL20, Human
mitochondrial ribosomal protein L20

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029039
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0032767; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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