Gene:  MPL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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In 1990 an oncogene v-mpl was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue named c-mpl was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl thrombopoietin was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene CD110 is a 635 amino acid transmembrane domain with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases as well as the STAT family the MAPK family the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015934
Info:   Same primer pair as used in probe assay qHsaCIP0030268
List Price:    $130.00
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PrimePCR™ Probe Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030268
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021241
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015934; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Copy Number Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000501
Info:   FAM; 69nt amplicon; exonic; FAM version of MPL (dHsaCP2506352)
 
Fluorophore:   FAM
List Price:    $240.00
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PrimePCR™ ddPCR™ Mutation Assay: MPL p.W515L, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500572
Info:   64nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $395.00
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PrimePCR™ ddPCR™ Mutation Assay: MPL WT for p.W515L, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500573
Info:   64nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:    $395.00
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PrimePCR™ ddPCR™ Copy Number Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506352
Info:   HEX; 69nt amplicon; exonic; HEX version of MPL (dHsaCP1000501)
 
Fluorophore:   HEX
List Price:    $240.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053848
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030268; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MPL, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053849
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030268; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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PrimePCR™ ddPCR™ Mutation Assay:MPL p.S505N, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503748
Info:   61nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $325.00
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PrimePCR™ ddPCR™ Mutation Assay:MPL WT for p.S505N, Human
myeloproliferative leukemia virus oncogene

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503749
Info:   61nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $325.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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