Gene:  MED27, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex which along with TFIID is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq Dec 2011]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: MED27, Human
mediator complex subunit 27

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038104
Info:   Same primer pair as used in probe assay qHsaCEP0040827
List Price:    $125.00
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PrimePCR™ Probe Assay: MED27, Human
mediator complex subunit 27

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040827
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MED27, Human
mediator complex subunit 27

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006404
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0038104; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MED27, Human
mediator complex subunit 27

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031120
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040827; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MED27, Human
mediator complex subunit 27

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031121
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040827; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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