Gene:  MECP2, Human

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DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2 MBD1 MBD2 MBD3 and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins with the exception of MBD3 is capable of binding specifically to methylated DNA. MECP2 MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq Jul 2009]

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PrimePCR™ SYBR® Green Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038147
Info:   Same primer pair as used in probe assay qHsaCEP0040870
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PrimePCR™ Probe Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040870
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006422
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0038147; exonic
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PrimePCR™ ddPCR™ Copy Number Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000579
Info:   FAM; 76nt amplicon; crosses exon-intron junction; FAM version of MECP2 (dHsaCP2506535)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506535
Info:   HEX; 76nt amplicon; crosses exon-intron junction; HEX version of MECP2 (dHsaCP1000579)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031156
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040870; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MECP2, Human
methyl CpG binding protein 2 (Rett syndrome)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031157
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040870; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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