Gene:  ME1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a cytosolic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme the reversible oxidative decarboxylation of malate links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ME1, Human
malic enzyme 1, NADP(+)-dependent, cytosolic

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015244
Info:   Same primer pair as used in probe assay qHsaCIP0029802
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PrimePCR™ Probe Assay: ME1, Human
malic enzyme 1, NADP(+)-dependent, cytosolic

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029802
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ME1, Human
malic enzyme 1, NADP(+)-dependent, cytosolic

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020665
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015244; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: ME1, Human
malic enzyme 1, NADP(+)-dependent, cytosolic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052982
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029802; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: ME1, Human
malic enzyme 1, NADP(+)-dependent, cytosolic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052983
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029802; Intron-spanning
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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