Gene:  MCFD2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LAMN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Jun 2010]

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PrimePCR™ SYBR® Green Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005775
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047420
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054680
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054680
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5003512
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005775; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011757
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047420; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041824
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054680; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MCFD2, Human
multiple coagulation factor deficiency 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041825
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054680; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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