Gene:  MCF2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq Jan 2010]

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PrimePCR™ SYBR® Green Assay: MCF2, Human
MCF.2 cell line derived transforming sequence

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042894
Info:   Same primer pair as used in probe assay qHsaCEP0050156
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PrimePCR™ SYBR® Green Assay: MCF2, Human
MCF.2 cell line derived transforming sequence

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007538
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PrimePCR™ Probe Assay: MCF2, Human
MCF.2 cell line derived transforming sequence

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050156
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PrimePCR™ ddPCR™ Mutation Assay:MCF2 p.V443A, Human
MCF.2 cell line derived transforming sequence

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501874
Info:   91nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MCF2 WT for p.V443A, Human
MCF.2 cell line derived transforming sequence

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501875
Info:   91nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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