Gene:  MANF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region including a specific mutation that changes the previously numbered codon 50 from ATG to AGG have been reported in a variety of solid tumors; however these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq Jun 2010]

PrimePCR™ SYBR® Green Assay: MANF, Human
mesencephalic astrocyte-derived neurotrophic factor

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003210
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: MANF, Human
mesencephalic astrocyte-derived neurotrophic factor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045243
Info:   Same primer pair as used in probe assay qHsaCEP0052504
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PrimePCR™ Probe Assay: MANF, Human
mesencephalic astrocyte-derived neurotrophic factor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052504
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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