Gene:  MAN2B1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an enzyme that hydrolyzes terminal non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa 42 kDa (D) and 13/15 kDa (E). Next the 70 kDa peptide is further processed into three peptides (A B and C). The A B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2010]

PrimePCR™ SYBR® Green Assay: MAN2B1, Human
mannosidase, alpha, class 2B, member 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043028
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050290
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PrimePCR™ SYBR® Green Assay: MAN2B1, Human
mannosidase, alpha, class 2B, member 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007081
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PrimePCR™ Probe Assay: MAN2B1, Human
mannosidase, alpha, class 2B, member 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050290
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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