Gene:  LOXHD1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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This gene encodes a highly conserved protein consisting entirely of PLAT (polycystin/lipoxygenase/alpha-toxin) domains thought to be involved in targeting proteins to the plasma membrane. Studies in mice show that this gene is expressed in the mechanosensory hair cells in the inner ear and mutations in this gene lead to auditory defects indicating that this gene is essential for normal hair cell function. Screening of human families segregating deafness identified a mutation in this gene which causes DFNB77 a progressive form of autosomal-recessive nonsyndromic hearing loss (ARNSHL). Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: LOXHD1, Human
lipoxygenase homology domains 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0008886
Info:   Same primer pair as used in probe assay qHsaCIP0026982
List Price:    $125.00
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PrimePCR™ Probe Assay: LOXHD1, Human
lipoxygenase homology domains 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026982
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LOXHD1, Human
lipoxygenase homology domains 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015977
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0008886; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LOXHD1, Human
lipoxygenase homology domains 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048214
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026982; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LOXHD1, Human
lipoxygenase homology domains 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048215
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026982; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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