Gene:  KPNA2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae) which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047532
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054792
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012953
List Price:    $125.00
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PrimePCR™ Probe Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054792
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019003
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012953; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189247
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047532; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192185
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054792; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: KPNA2, Human
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192186
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054792; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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