Gene:  KIT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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Control and Reference Assays for Real-Time PCR

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors mast cell disease acute myelogenous lukemia and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: KIT, Human
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0008692
Info:   Same primer pair as used in probe assay qHsaCIP0026913
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PrimePCR™ Probe Assay: KIT, Human
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026913
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PrimePCR™ ddPCR™ Copy Number Assay: KIT, Human
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000032
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KIT p.D816V, Human
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000023
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KIT WT for p.D816V, Human
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000024
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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