Gene:  KDELR3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal usually lys-asp-glu-leu (KDEL) in animal cells and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes and binds the tetrapeptide-containing protein and returns it to the ER. In yeast the sorting receptor encoded by a single gene ERD2 is a seven-transmembrane protein. Unlike yeast several human homologs of the ERD2 gene constituting the KDEL receptor gene family have been described. KDELR3 was the third member of the family to be identified and it encodes a protein highly homologous to KDELR1 and KDELR2 proteins. Two transcript variants of KDELR3 that arise by alternative splicing and encode different isoforms of KDELR3 receptor have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042749
Info:   Same primer pair as used in probe assay qHsaCEP0050011
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007933
List Price:    $130.00
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PrimePCR™ Probe Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050011
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007599
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042749; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015252
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007933; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033510
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050011; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: KDELR3, Human
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033511
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050011; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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