Gene:  JAK3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: JAK3, Human
Janus kinase 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012535
Info:   Same primer pair as used in probe assay qHsaCIP0028483
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PrimePCR™ Probe Assay: JAK3, Human
Janus kinase 3

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028483
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 p.M511I, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501476
Info:   75nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 WT for p.M511I, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501477
Info:   75nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 p.A573V, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501604
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 WT for p.A573V, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501605
Info:   98nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 p.A572V, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503276
Info:   96nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 WT for p.A572V, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503277
Info:   96nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 p.M511T, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505044
Info:   76nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK3 WT for p.M511T, Human
Janus kinase 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505045
Info:   76nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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