Gene:  JAK2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: JAK2, Human
Janus kinase 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042701
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049963
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PrimePCR™ SYBR® Green Assay: JAK2, Human
Janus kinase 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006487
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PrimePCR™ Probe Assay: JAK2, Human
Janus kinase 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049963
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PrimePCR™ ddPCR™ Copy Number Assay: JAK2, Human
Janus kinase 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000500
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: JAK2 p.V617F, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000061
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: JAK2 WT for p.V617F, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000062
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.G562G, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501530
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.G562G, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501531
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.N533D, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501572
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.N533D, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501573
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.G571S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502546
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.G571S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502547
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.R683G, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502708
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.R683G, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502709
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.Y570Y, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502826
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.Y570Y, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502827
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.I166T, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503144
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.I166T, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503145
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.R683S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503290
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.R683S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503291
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.N1108S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503350
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.N1108S, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503351
Info:   97nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.C618R, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504006
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.C618R, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504007
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 p.T514A, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506046
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:JAK2 WT for p.T514A, Human
Janus kinase 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506047
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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