Gene:  ITCH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Mar 2012]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057312
Info:   Same primer pair as used in probe assay qHsaCEP0058417
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PrimePCR™ SYBR® Green Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010146
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PrimePCR™ Probe Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058417
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013865
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0057312; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016872
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010146; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500413
Info:   FAM; 80nt amplicon; intronic; FAM version of ITCH (dHsaCP2506671)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506671
Info:   HEX; 80nt amplicon; intronic; HEX version of ITCH (dHsaCP2500413)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046040
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0058417; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: ITCH, Human
itchy E3 ubiquitin protein ligase homolog (mouse)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046041
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0058417; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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