Gene:  INCENP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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In mammalian cells 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' or transiently interacting proteins (reviewed by Choo 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139) CENPB (MIM 117140) CENPC1 (MIM 117141) and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g. MIM 600112); CliPs (e.g. MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke 1991 [PubMed 1860899]) the initial members of the passenger protein group display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase the proteins are located within the midbody in the intercellular bridge where they are discarded after cytokinesis (Cutts et al. 1999 [PubMed 10369859]).[supplied by OMIM Mar 2008]

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PrimePCR™ SYBR® Green Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045453
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052714
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010103
List Price:    $125.00
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PrimePCR™ Probe Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052714
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009992
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045453; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016841
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010103; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038294
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052714; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: INCENP, Human
inner centromere protein antigens 135/155kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038295
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052714; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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