Gene:  IDH3G, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits one beta subunit and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described but only some of their full length natures have been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042263
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049526
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013684
List Price:    $130.00
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PrimePCR™ Probe Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049526
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007210
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042263; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019509
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013684; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032732
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049526; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: IDH3G, Human
isocitrate dehydrogenase 3 (NAD+) gamma

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032733
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049526; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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