Gene:  IDH3A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits one beta subunit and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046378
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053639
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015549
List Price:    $125.00
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PrimePCR™ Probe Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053639
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5010829
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046378; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020914
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015549; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5039968
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0053639; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: IDH3A, Human
isocitrate dehydrogenase 3 (NAD+) alpha

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5039969
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0053639; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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