Gene:  IDE, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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This gene encodes a zinc metallopeptidase that degrades intracellular insulin and thereby terminates insulins activity as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon amylin bradykinin and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space cell membrane peroxisome and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq Sep 2009]

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PrimePCR™ SYBR® Green Assay: IDE, Human
insulin-degrading enzyme

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0036464
Info:   Same primer pair as used in probe assay qHsaCIP0039188
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PrimePCR™ Probe Assay: IDE, Human
insulin-degrading enzyme

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0039188
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PrimePCR™ ddPCR™ Copy Number Assay: IDE, Human
insulin-degrading enzyme

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000441
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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