Gene:  HYOU1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors especially in breast tumors and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein which is only 3 amino acids shorter than the mature protein in the ER is thought to have a housekeeping function in the cytosol. In rat this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq Oct 2008]

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PrimePCR™ SYBR® Green Assay: HYOU1, Human
hypoxia up-regulated 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0019708
Info:   Same primer pair as used in probe assay qHsaCIP0032511
List Price:    $125.00
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PrimePCR™ Probe Assay: HYOU1, Human
hypoxia up-regulated 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0032511
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HYOU1, Human
hypoxia up-regulated 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023437
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0019708; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HYOU1, Human
hypoxia up-regulated 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057012
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0032511; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HYOU1, Human
hypoxia up-regulated 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057013
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0032511; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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