Gene:  HUS1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a component of an evolutionarily conserved genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC) which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2011]

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PrimePCR™ SYBR® Green Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044682
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051944
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006256
List Price:    $125.00
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PrimePCR™ Probe Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051944
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009308
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044682; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014323
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006256; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036928
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051944; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HUS1, Human
HUS1 checkpoint homolog (S. pombe)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036929
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051944; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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