Gene:  HSD11B2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme encoded by this gene has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor such as the placenta and testis it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq Feb 2010]

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PrimePCR™ SYBR® Green Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002824
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047172
Info:   Same primer pair as used in probe assay qHsaCEP0054433
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054433
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001452
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002824; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011529
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047172; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041368
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054433; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HSD11B2, Human
hydroxysteroid (11-beta) dehydrogenase 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041369
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054433; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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