Gene:  HSBP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1) is a 76-amino-acid protein that binds to heat shock factor 1(HSF1) which is a transcription factor involved in the HS response. During HS response HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HSBP1, Human
heat shock factor binding protein 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014176
Info:   Same primer pair as used in probe assay qHsaCIP0029242
List Price:    $125.00
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PrimePCR™ Probe Assay: HSBP1, Human
heat shock factor binding protein 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029242
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HSBP1, Human
heat shock factor binding protein 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019884
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0014176; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HSBP1, Human
heat shock factor binding protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052020
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029242; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: HSBP1, Human
heat shock factor binding protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052021
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029242; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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