Gene:  HPRT1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a transferase which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq Jun 2009]

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PrimePCR™ SYBR® Green Assay: HPRT1, Human
hypoxanthine phosphoribosyltransferase 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016375
Info:   Same primer pair as used in probe assay qHsaCIP0030549
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PrimePCR™ Probe Assay: HPRT1, Human
hypoxanthine phosphoribosyltransferase 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030549
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HPRT1, Human
hypoxanthine phosphoribosyltransferase 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189658
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016375; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: HPRT1, Human
hypoxanthine phosphoribosyltransferase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192871
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030549; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: HPRT1, Human
hypoxanthine phosphoribosyltransferase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192872
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030549; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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