Gene:  HLA-DRB5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3 DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1 otherwise it is omitted. There are 4 related pseudogenes: DRB2 DRB6 DRB7 DRB8 and DRB9. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: HLA-DRB5, Human
major histocompatibility complex, class II, DR beta 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013959
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: HLA-DRB5, Human
major histocompatibility complex, class II, DR beta 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019719
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013959; Intron-spanning
List Price:    $130.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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