Gene:  GTF2A2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA TFIIB (MIM 189963) TFIID (MIM 313650) TFIIE (MIM 189962) TFIIF (MIM 189968) TFIIG/TFIIJ and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520) respectively and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al. 1995 [PubMed 7724559]).[supplied by OMIM Mar 2008]

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PrimePCR™ SYBR® Green Assay: GTF2A2, Human
general transcription factor IIA, 2, 12kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013726
Info:   Same primer pair as used in probe assay qHsaCIP0029009
List Price:    $125.00
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PrimePCR™ Probe Assay: GTF2A2, Human
general transcription factor IIA, 2, 12kDa

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029009
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GTF2A2, Human
general transcription factor IIA, 2, 12kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019541
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013726; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GTF2A2, Human
general transcription factor IIA, 2, 12kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051642
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029009; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GTF2A2, Human
general transcription factor IIA, 2, 12kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051643
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029009; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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