Gene:  GSTA3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic carcinogenic and pharmacologically active electrophilic compounds. At present eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha kappa mu omega pi sigma theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However during evolution this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified but its full length sequence has not been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047032
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054293
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020629
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054293
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011407
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047032; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023574
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020629; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041124
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054293; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: GSTA3, Human
glutathione S-transferase alpha 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041125
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054293; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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