Gene:  Gnas, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009]

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PrimePCR™ SYBR® Green Assay: Gnas, Rat
GNAS isoform GNASL

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qRnoCED0052955
List Price:    $125.00
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PrimePCR™ Probe Assay: Gnas, Rat
GNAS isoform GNASL

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qRnoCEP0046775
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Gnas, Rat
GNAS isoform GNASL

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dRnoEG5135050
Info:   EG; Same primer pair as used in qPCR assay qRnoCED0052955; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Gnas, Rat
GNAS isoform GNASL

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5166078
Info:   FAM; Same primer pair and probe as used in qPCR assay qRnoCEP0046775; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Gnas, Rat
GNAS isoform GNASL

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5166079
Info:   HEX; Same primer pair and probe as used in qPCR assay qRnoCEP0046775; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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