Gene:  GNAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This locus has a highly complex imprinted expression pattern. It gives rise to maternally paternally and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus and the antisense transcript are paternally expressed noncoding RNAs and may regulate imprinting in this region. In addition one of the transcripts contains a second overlapping ORF which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed which results in different forms of the stimulatory G-protein alpha subunit a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a pseudohypoparathyroidism type 1b Albright hereditary osteodystrophy pseudopseudohypoparathyroidism McCune-Albright syndrome progressive osseus heteroplasia polyostotic fibrous dysplasia of bone and some pituitary tumors. [provided by RefSeq Mar 2009]

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PrimePCR™ SYBR® Green Assay: GNAS, Human
GNAS complex locus

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013652
Info:   Same primer pair as used in probe assay qHsaCIP0028974
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PrimePCR™ Probe Assay: GNAS, Human
GNAS complex locus

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028974
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PrimePCR™ ddPCR™ Copy Number Assay: GNAS, Human
GNAS complex locus

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000398
Info:   63nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: GNAS p.R201C, Human
GNAS complex locus

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500562
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: GNAS WT for p.R201C, Human
GNAS complex locus

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500563
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:GNAS p.R374C, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501138
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:GNAS WT for p.R374C, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501139
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:GNAS p.R201H, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501324
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:GNAS WT for p.R201H, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501325
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:GNAS p.D141G, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501948
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:GNAS WT for p.D141G, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501949
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:GNAS p.R201S, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503808
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:GNAS WT for p.R201S, Human
GNAS complex locus

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503809
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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