Gene:  GBE1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-14-linked glucosyl units from the outer end of a glycogen chain to an alpha-16 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and consequently in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: GBE1, Human
glucan (1,4-alpha-), branching enzyme 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007532
Info:   Same primer pair as used in probe assay qHsaCIP0026504
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PrimePCR™ Probe Assay: GBE1, Human
glucan (1,4-alpha-), branching enzyme 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026504
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: GBE1, Human
glucan (1,4-alpha-), branching enzyme 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015158
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007532; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: GBE1, Human
glucan (1,4-alpha-), branching enzyme 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5047368
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026504; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: GBE1, Human
glucan (1,4-alpha-), branching enzyme 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5047369
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026504; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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