Gene:  FBXW7, Human

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This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box) which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains Fbls containing leucine-rich repeats and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30 and belongs to the Fbws class; in addition to an F-box this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2012]

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PrimePCR™ SYBR® Green Assay: FBXW7, Human
F-box and WD repeat domain containing 7

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017122
Info:   Same primer pair as used in probe assay qHsaCIP0031037
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PrimePCR™ Probe Assay: FBXW7, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031037
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PrimePCR™ ddPCR™ Copy Number Assay: FBXW7, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000426
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FBXW7 p.R465C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500506
Info:   80nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: FBXW7 WT for p.R465C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500507
Info:   80nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R505L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500694
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R505L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500695
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.Y545C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500852
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.Y545C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500853
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.S582L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500960
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.S582L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500961
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R505C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501126
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R505C, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501127
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R465R, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501358
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R465R, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501359
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R224*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501848
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R224*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501849
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R658*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502136
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R658*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502137
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.G423V, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502366
Info:   67nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.G423V, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502367
Info:   67nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.E192A, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502676
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.E192A, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502677
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R479L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502798
Info:   118nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R479L, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502799
Info:   118nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R278*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503028
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R278*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503029
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R13R, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503170
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R13R, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503171
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R13*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504344
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R13*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504345
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R367*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504422
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R367*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504423
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.E369*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504432
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.E369*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504433
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R479Q, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504578
Info:   118nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R479Q, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504579
Info:   118nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R465H, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505080
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R465H, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505081
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.E693E, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505200
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.E693E, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505201
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R393*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505474
Info:   99nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R393*, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505475
Info:   99nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R689W, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505548
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R689W, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505549
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 p.R505G, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505658
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FBXW7 WT for p.R505G, Human
F-box and WD repeat domain containing 7

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505659
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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