Gene:  FARP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain a Dbl homology domain and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: FARP1, Human
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056826
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057931
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PrimePCR™ SYBR® Green Assay: FARP1, Human
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021439
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PrimePCR™ Probe Assay: FARP1, Human
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057931
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PrimePCR™ ddPCR™ Mutation Assay:FARP1 p.S383L, Human
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503410
Info:   61nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:FARP1 WT for p.S383L, Human
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503411
Info:   61nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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