Gene:  F9, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions membrane phospholipids and factor VIII. Alterations of this gene including point mutations insertions and deletions cause factor IX deficiency which is a recessive X-linked disorder also called hemophilia B or Christmas disease. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: F9, Human
coagulation factor IX

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042895
Info:   Same primer pair as used in probe assay qHsaCEP0050157
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PrimePCR™ SYBR® Green Assay: F9, Human
coagulation factor IX

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014849
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PrimePCR™ Probe Assay: F9, Human
coagulation factor IX

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050157
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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