Gene:  F2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047410
Info:   Same primer pair as used in probe assay qHsaCEP0054670
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PrimePCR™ SYBR® Green Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009298
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054670
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011749
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047410; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016285
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009298; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041808
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054670; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: F2, Human
coagulation factor II (thrombin)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5041809
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054670; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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