Gene:  ERBB2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported with the most common allele Ile654/Ile655 shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers including breast and ovarian tumors. Alternative splicing results in several additional transcript variants some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ERBB2, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045039
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052301
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PrimePCR™ SYBR® Green Assay: ERBB2, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012766
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PrimePCR™ Probe Assay: ERBB2, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052301
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PrimePCR™ ddPCR™ Copy Number Assay: ERBB2, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000116
Info:   66nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.D769Y, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501076
Info:   59nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.D769Y, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501077
Info:   59nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.S310F, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501414
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.S310F, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501415
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.V777L, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501544
Info:   59nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.V777L, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501545
Info:   59nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.G776V, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503826
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.G776V, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503827
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.V842I, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504516
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.V842I, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504517
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.R678Q, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504764
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.R678Q, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504765
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.L755S, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505134
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.L755S, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505135
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 p.E971G, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505920
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ERBB2 WT for p.E971G, Human
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505921
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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