Gene:  EPHA3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: EPHA3, Human
EPH receptor A3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036310
Info:   Same primer pair as used in probe assay qHsaCEP0039034
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PrimePCR™ SYBR® Green Assay: EPHA3, Human
EPH receptor A3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0057450
Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCIP0058555
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PrimePCR™ Probe Assay: EPHA3, Human
EPH receptor A3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039034
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PrimePCR™ Probe Assay: EPHA3, Human
EPH receptor A3

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0058555
Info:   Recommended - best negative control
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 p.S229S, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500826
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 WT for p.S229S, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500827
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 p.N85S, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504230
Info:   72nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 WT for p.N85S, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504231
Info:   72nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 p.L122M, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505214
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 WT for p.L122M, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505215
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 p.R769H, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505276
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:EPHA3 WT for p.R769H, Human
EPH receptor A3

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505277
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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