Gene:  EIF5B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Accurate initiation of translation in eukaryotes is complex and requires many factors some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1 IF2 IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: EIF5B, Human
eukaryotic translation initiation factor 5B

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006114
Info:   Same primer pair as used in probe assay qHsaCIP0026027
List Price:    $125.00
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PrimePCR™ Probe Assay: EIF5B, Human
eukaryotic translation initiation factor 5B

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026027
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EIF5B, Human
eukaryotic translation initiation factor 5B

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014205
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006114; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF5B, Human
eukaryotic translation initiation factor 5B

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046518
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026027; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF5B, Human
eukaryotic translation initiation factor 5B

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046519
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026027; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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